Rotor Gene 6000 Real-Time PCR Machine Features: • Unique centrifugal rotary format • Uniformity: ± 0.01°C • Resolution: 0.02°C (smallest programmable and reported thermal increment) • 5-Plex (Green, Yellow, Orange, Red, Crimson) fluorescence channels • High resolution melt (HRM) application • Holds 36 x0.2ml tubes, 72 x 0.1ml strip tubes or 100 Gene-Disc • Software: Build-in extensive analysis, graphing and statistical functions. Unlimited use software license for Windows XP, Pentium IV (2GHz) or higher PC. Rotor Gene 3000 Real-Time PCR Machine Features: • Unique centrifugal rotary format • Excitation Source: LED high power diodes, 470nm, 530nm, 585nm and 625nm • Detection Filters: 510nm (FAM), 555nm (JOE), 610nm (ROX) and 660nm high-pass (Cy5) • Holds 36 x0.2ml tubes or 72 x 0.1ml strip tubes • Software: Build-in extensive analysis, graphing and statistical functions. Glad2teach Math Tricks. Use of the machines in the Molecular Biology Facility • The Rotor-Gene machines are in high demand therefore there is an (external website). Plan ahead, book time slots and stick to them. • Users are responsible for supplying all their own plastic ware and reagents. 0.1ml tubes and Gene-Disc 100 are produced by Corbett especially for the Rotor-Gene.

• Save files in your own folder in the 'Rotor Gene Run File' folder in the local disk (C:). The computer is also linked to the network for easy transfer of your data to the server. Tips and addition information • The Rotor-Gene machines support reaction volumes from 5 µl to 100 µl (rotor and protocol dependent). Typical reaction volume is 20µl.

QIAGEN®, QIAgility ®, EpiTect, HotStarTaq ®, Quantiscript, QuantiTect ®, Rotor-Gene, Rotor-Disc. 4.7 Software installation 4-6 4.8 Software version 4-9. This software is the most powerful tool currently available for analysis of HRM data from the Rotor-Gene Q or Rotor-Gene 6000 cycler. Rotor-Gene Q software.

• The use of a normalisation dye, such as ROX reference dye, is optional on the Rotor-Gene. • Many companies supply master mixes for real-time PCR which can decrease the chance of pipetting errors in this highly sensitive method. • If you would like to learn more about the real-time PCR experimental design and data analysis, please contact Dr. Donna Lai, the Molecular Biology Officer. Bioanalyzer, Real-time RT-PCR workshops are conducted occasionally during the year. Please contact Dr.

Donna Lai for details.

The Rotor-Gene 6000 is unlike any other real-time system. It uses a unique centrifugal rotary design, ideal for even the most demanding requirements of real-time thermo-optical analyses. What sets the Rotor-Gene apart is its amazing precision; the result of near-perfect well-to-well thermal and optical uniformity. Its design also enables a fast data acquisition rate, a capability now proving to be essential for advanced applications such as HRM (high-resolution melt). Features: - It supports the broadest range of applications, including quantification, HRM (high-resolution melt), end-point, association/disassociation (i.e. Melting/annealing) kinetics, and nucleic acid concentration measurement - Included software supports the most real-time data analysis methods - It has the greatest optical range, with 6 channels spanning UV to infra-red wavelengths - It uses the widest range of reaction tube formats.